小腸実験かいようの走査電子顕微鏡学的研究　第二編　　インドメタシンかいようにおける表面微細形態の観察

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  • SCANNING ELECTRON MICROSCOPIC STUDIES ON THE EXPERIMENTAL ULCER OF THE RAT SMALL INTESTINE
  • 第二編インドメサシン潰瘍における表面微細形態の観察

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Scanning electron microscopic studies were carried out to clarify the ultrastructural change of indomethacin-induced small intestinal lesions. Male Sprague-Dawley rats weighing 250-300g were used for the studies. Indomethacin of 20mg/kg was administered to rats by subcutaneous injection. Various stages of the indomethacin-induced lesions were investigated and following results were obtained. (1) After 1hr of indomethacin administration, initial lesions could be detected as small reddish spots by a dissecting microscope. Red blood cells remained in the capillary vessels of villi after irrigation with the Ringer solution. Irregularly distorted villi and desquamation of epithelial cells were observed by SEM. These findings were suggested that the microcirculation of villi was disturved. (2) After 2-3hrs, intestinal lesions could be clearly detected macroscopically as reddish milliary-sized spots. In the reddish spots, exposed villous cores and rounded epithelial cells between villous cores were observed. (3) After 24hrs, multiple shallow ulcers were formed at the distal ileum. Exposed villous cores and distorted villi which were similar to the initial lesions of the intestine were observed in the marginal zone of ulcers. The ulcer reached the maximum size on the third day of experiment. (4) After 10 days, regenerated villi were arranged radially to the ulcer margin, and a sheet of regenerated cell layer extended over the ulcer base. Irregularly rounded or extended cells with sparse microvilli were observed along the growing epithelial edge. (5) The goblet cells secreting a small amount of mucus were seen on the third day and on that day the mucosa was maximally damaged. However, frequently a plenty amount of mucus was seen to be released from goblet cells in the early stage of damage or healing stage.

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