Detection of cyclic AMP levels and cyclic AMP-receptor protein in mycelia of Rhizoctonia solani forming sclerotia.

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  • イネ紋枯病菌の菌核形成時に菌糸中に存在する環状アデニル酸とその結合蛋白質の検出
  • イネ紋枯病菌の菌核形成時に菌糸中に存在する環状アデニル酸とその結合蛋白質の検出〔英文〕
  • イネ モンガレビョウキン ノ キンカク ケイセイジ ニ キンシチュウ ニ ソン

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Rhizoctonia solani isolates capable of forming sclerotia accumulated two or three times higher amounts of adenosine 3', 5'-cyclic monophosphate (cAMP) in mycelial cells comparing with those incapable of forming sclerotia. The addition of adenine and L-cysteine to culture media was effective to inhibit formation of sclerotia. Adenine inhibited adenylate cyclase activity, but not phosphodiesterase activity. L-cysteine showed no effect on both adenylate cyclase and phosphodiesterase activities. A single cAMP-receptor protein was detected by photoaffinity labeling with 8-N3-cAMP in mycelial extracts of all isolates of R. solani tested. The protein had an apparent molecular weight of 50, 000 and co-chromatographed on a DEAE-Sephacel column with cAMP-dependent protein kinase. The result indicated that the 50, 000-dalton protein may be a regulatory subunit of protein kinase with capacity to bind cAMP.

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