The Behavior of Lux-marked Ralstonia solanacearum in Grafted Tomato Cultivars Resistant or Susceptible to Bacterial Wilt.

DOI Web Site 20 Citations 28 References
  • HIKICHI Yasufumi
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture,Kochi University
  • NAKAZAWA-NASU Yoshiko
    Iwate Biotechnology Research Center Tochigi Prefectural Agricultural Experimental Station
  • KITANOSONO Shinobu
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture,Kochi University
  • SUZUKI Kazumi
    Iwate Biotechnology Research Center
  • OKUNO Tetsuro
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture,Kochi University

Bibliographic Information

Other Title
  • 青枯病抵抗性と感受性トマト品種による接木トマトにおける<i>lux</i>標識<i>Ralstonia solanacearum</i>の挙動
  • Behavior of Lux-marked Ralstonia solanacearum in Grafted Tomato Cultivars Resistant or Susceptible to Bacterial Wilt
  • The behavior ofgenetically lux-marked Ralstonia solanacearum in grafted tomato cultivars resistant orsusceptible to bacterial wilt.

Search this article

Abstract

Ralstonia solanacearum OE1-1 was transformed with pNP126 carrying a luxCDABE operon of Vibrio fisheri and a promoter region derived from the genomic DNA of Burkholderia glumae. Intensity of bioluminescence from YN5 thus obtained positively correlated with growth of the bacteria from the lag phase to the stationary phase in vitro. In this study, we used the VIM camera equipped with the ARGUS 50 to successively observe bioluminescence and the development of bacterial wilt in singly and doubly grafted tomato plants in various combinations of the resistant LS-89 and the susceptible Oogata-Fukuju. The degree of bacterial growth in the roots and collars was one of the determinants for the induction of bacterial wilt in tomato plants. Resistance of LS-89 resulted from suppression of bacterial proliferation in the roots and stems below the first leaf. In grafted tomato plants using LS-89 as the rootstock, all rootstocks were latently infected with the bacteria. The bacteria were also recovered from susceptible scions even though the plants did not wilt. In some plants, the bacteria proliferated in the susceptible scions. These plants wilted heavily. Taken together, suppression of bacterial proliferation in the roots and the stems below the first leaf of resistant rootstocks affected the bacterial growth in the susceptible scions and quantative control of resistance against development of the disease.

Journal

Citations (20)*help

See more

References(28)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top