Some Physicochemical and Serological Properties of Konjak Mosaic Virus and Dasheen Mosaic Virus.

DOI JASI Web Site
  • SHIMOYAMA Jun
    Konjak Branch Station, Gunma Agricultural Experiment Station
  • HANADA Kaoru
    National Agriculture Research Center Kyushu National Agricultural Experiment Station
  • TSUDA Shinya
    NODAI Research Institute, Tokyo University of Agriculture Plant Biotechnology Institute, Ibaraki Agricultural Center
  • KAMEYA-IWAKI Mituro
    Faculty of Agriculture, Yamaguchi University
  • GUNJI Takashi
    Konjak Branch Station, Gunma Agricultural Experiment Station

Bibliographic Information

Other Title
  • コンニャクモザイクウイルスとサトイモモザイクウイルスの理化学的性状と血清学的性質
  • コンニャク モザイク ウイルス ト サトイモ モザイク ウイルス ノ リカガク

Search this article

Description

The physicochemical and serological properties of konjak mosaic virus (KMV) and dasheen mosaic virus (DasMV) were studied. The molecular weight (MW) of nucleic acid of each virus was estimated to be 3.21×106 for KMV, and 3.42×106 for DasMV under denaturing conditions. While KMV had a single coat protein with MW of 35.5K, DasMV had two coat proteins with MWs of 32.5K and 40.0K. The 32.5K polypeptide was assumed to be derived from proteolytic degradation of the 40.0K protein, since both the proteins showed almost identical peptide patterns when analyzed by reverse phase high performance chromatography after treatment with Lysyl endopeptidase. ELISA showed that KMV was serologically related to soybean mosaic virus (SoyMV) and watermelon mosaic virus-2 (WMV-2) but unrelated to other six potyviruses including DasMV. Also, SoyMV and WMV-2 reacted with anti-KMV antibody by double immunodiffusion and western blotting analyses.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top