糸状菌に対するポリオキシンの影響 (IX)

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  • Effect of polyoxin on fungi. Ultrastructural and cytochemical analyses of polyoxin-treated hyphae of Alternaria kikuchiana Tanaka.
  • 糸状菌に対するポリオキシンの影響-9-ポリオキシン処理したAlternaria kikuchiana Tanaka菌糸の微細構造的・細胞化学的観察〔英文〕
  • シジョウキン ニ タイスル ポリオキシン ノ エイキョウ 9 ポリオキシン シ
  • Ultrastructural and Cytochemical Analyses of Polyoxin-treated Hyphae of <i>Alternaria kikuchiana</i> Tanaka

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Polyoxin-B-treated and untreated (control) hypae of Alternaria kikuchiana Tanaka were first degraded with 2N NaOH, 1N H2SO4, and digestive enzymes, and then morphological alterations of the cell walls were investigated by cytochemical methods and electron microscopy. A pronounced difference between control and treated hyphae was seen after sequential treatment with NaOH-H2SO4-NaOH. Inner wall layers of controls were transformed into numerous microfibrils; these were identified, in turn, by chitinase and Lytic enzyme digestions as chitin and β-1, 3-glucan intermixed with protein. But these same treatments failed to structurally degrade the walls of polyoxin-treated hyphae. In the controls, only the thin outer wall layer showed any resistance to the treatment, but in polyoxin-treated hyphae the entire cell wall, including septae and walls of intracellular hypha, was resistant to a sequence of the degradative treatments. Cytochemical tests revealed that both control and polyoxin-treated hyphae, which originally contained chitin, no longer responded positively to tests for chitin after chitinase or HCl treatment following treatment with the NaOH-H2SO4-NaOH sequence. This study suggests two possibilities i) cell walls, especially inner wall layers, of control and polyoxin-treated hyphae may have different structural constituents, and ii) melanin-like pigments in inner cell walls may be associated with the resistance of polyoxin-treated hyphae to lysis by digestive enzymes.

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