Detection of Tobacco Necrotic Dwarf Virus Antigen in Plant Tissues by Fluorescent Antibody Staining

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  • IMAIZUMI Seiko
    Central Research Institute, The Japan Tobacco & Salt Public Corporation
  • KUBO Susumu
    Central Research Institute, The Japan Tobacco & Salt Public Corporation

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Other Title
  • ケイ光抗体法による植物組織内のタバコえそい縮ウイルス抗原の検出
  • 螢光抗体法による植物組織内のタバコえそ萎縮ウイルス抗原の検出
  • ケイコウ コウタイホウ ニヨル ショクブツ ソシキナイ ノ タバコ エソイシュ

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Abstract

Location of tobacco necrotic dwarf virus antigen in plant tissues was detected by the fluorescent antibody staining method.<br> The antigen was restricted to phloem tissue in tobacco, Physalis floridana and spinach. A method using polyethylene glycol 1000 for embedding tissues fixed with the Carnoy's fixative was proved to be rapid and effective to detect the specific fluorescence in tissue sections.

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