Detection of Fusarium spp. in Plants with Monoclonal Antibody.
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- ARIE Tsutomu
- The Institute of Physical and Chemical Research (RIKEN)
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- HAYASHI Yoshio
- Faculty of Agriculture, Meiji University
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- YONEYAMA Katsuyoshi
- Faculty of Agriculture, Meiji University
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- NAGATANI Akira
- The Institute of Physical and Chemical Research (RIKEN)
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- FURUYA Masaki
- Advanced Research Laboratory, Hitachi Ltd.
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- YAMAGUCHI Isamu
- The Institute of Physical and Chemical Research (RIKEN)
Bibliographic Information
- Other Title
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- モノクローナル抗体を用いた植物組織内のフザリウム菌検出法
- モノクローナル抗体を用いた植物組織内のフザリウム菌検出法〔英文〕
- モノクローナル コウタイ オ モチイタ ショクブツ ソシキナイ ノ フザリウム
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Abstract
A monoclonal antibody (MoAb) API9-2, which reacts specifically to members of the genus Fusarium, was tested for serological detection of F. oxysporum in plant tissue. Mycelia of F. oxysporum in infected stems, crowns, and roots of tomato and Japanese honewort plants were effectively detected by immunofluorescence assay (IFA) with the combination of MoAb API9-2 and FITC-conjugated anti-mouse IgM-sheep IgG indicating that IFA is a useful tool for the observation of behavior of Fusarium spp. in plant tissue. As another immunological assay with this MoAb, direct tissue-blotted immunobinding assay (DT-IBA) was applied to transverse sections from stems or crowns of tomato and cucumber plants. In this assay, samples of plants infected with F. oxysporum showed positive reaction within 4hr. In addition, the pathogen residing in vessels of infected, but symptomless plants was observed within 14 to 21 days after inoculation. Thus, DT-IBA is thought to be a practical method for quick and accurate diagnosis of Fusarium spp.
Journal
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- Japanese Journal of Phytopathology
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Japanese Journal of Phytopathology 61 (4), 311-317, 1995
The Phytopathological Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282681379282432
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- NII Article ID
- 110002732313
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- NII Book ID
- AN0019269X
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- COI
- 1:CAS:528:DyaK2MXotlGrs7w%3D
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- ISSN
- 18820484
- 00319473
- http://id.crossref.org/issn/00319473
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- NDL BIB ID
- 3630548
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed