A Sliced-Sheath Inoculation Method for Continuous Observation of Tissue Cells of Rice Infected with <i>Pyricularia oryzae</i> Cavara

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  • イネいもち病感染初期の連続観察のためのはく離葉しょう接種法
  • イネ イモチビョウ カンセン ショキ ノ レンゾク カンサツ ノ タメ ノ ハ

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We deviced a sliced-sheath inoculation method for continuous observation of leaf sheath cells infected with Pyricularia oryzae Cavara. By this method, the aspects of intracellular hyphal growth in the same tissues were observed successively without sectioning of tissues at various times after inoculation. The growth of infection hyphae in sliced-sheath cells was less than that in non-sliced-sheath cells at 32-40hr after inoculation. It might be due to the physiological changes of sheath cells resulted by slicing tissues. The method proposed here seems to be suitable for study of rice-Pyricularia oryzae interaction.

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