<i>Erwinia carotovora</i> subsp. <i>carotovora</i> EC1株の複数のポリガラクツロナーゼを生産するDNA領域の核酸塩基配列の決定

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  • Determination of Nucleotide Sequence of a Region Producing Multiple Polygalacturonases from Erwinia carotovora subsp. carotovora EC1.
  • Erwinia carotovora subsp.carotovora EC1株の複数のポリガラクツロナーゼを生産するDNA領域の核酸塩基配列の決定〔英文〕
  • Erwinia carotovora subsp.carotovora EC1

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DNA region consists of 1804 bases (b) was shown to produce four Polygalacturonases (Peh) in Erwinia carotovora subsp. carotovora (E.c.c.) EC1. The sequence of this region was determined in both directions. In this DNA fragment, there was one open reading frame (ORF1) that encodes for a protein of 402 amino acids with a calculated molecular weight of 42, 646 which is in the range of previously reported molecular weight of E.c.c. Peh. The sequence of the amino acids deduced from ORF1 showed 90% homology with that of reported E.c.c. Peh. A possible signal peptide of 26 amino acids was found. Several shorter ORF were also found from both strands. Possible role of remaining 595b for the production of four Peh was discussed.

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