<i>Rhizoctonia solani</i>菌糸融合第4群菌株のプラスミド様DNAの遺伝的類縁性

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  • Genetic Relatedness of Plasmid-Like DNA in Isolates from Anastomosis Group 4 of Rhizoctonia solani.
  • Rhizoctonia solani菌糸融合第4群菌株のプラスミド様DNAの遺伝的類縁性〔英文〕
  • Rhizoctonia solani キンシ ユウゴウ ダイ 4グン キンカブ

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Plasmid-like DNAs (plDNAs) were found associated with mitochondrial DNA fraction following CsCl-bisbenzimide density-gradient ultracentrifugation for 15 isolates belonging to the anastomosis group 4 (AG-4) of Rhizoctonia solani. These 15 isolates were divided into two classes based on the size of plDNAs observed. Four isolates contained 2.4kb plDNA and 2 of them carried an additional plDNA of 4.8kb. Eleven isolates contained 2.7kb plDNAs and 4 of them carried an additional plDNA of 5.4kb. All these plDNAs of 2.4kb and 2.7kb hybridized strongly with the probes of 2.7kb plDNAs, pRS64-1, -2 and -3. Restriction mapping of 2.4kb plDNAs revealed the presence of two types of maps. Two isolates carried both types of 2.4kb plDNAs. Restriction mapping of 2.7kb plDNAs revealed the presence of three types of maps. One isolate contained two types of 2.7kb plDNAs, and other isolate contained three types corresponding with those of pRS64-1, -2, and -3, respectively. The isolates in AG-4 of R. solani were classified into two groups based on the presence of 2.4 and 2.7kb plDNAs.

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