Some properties of pea stem necrosis virus isolated fro pea in Japan.
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- OSAKI Takeshi
- College ofAgriculture, University of Osaka Prefecture
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- OZAKI Kenji
- College ofAgriculture, University of Osaka Prefecture
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- INOUYE Tadao
- College ofAgriculture, University of Osaka Prefecture
Bibliographic Information
- Other Title
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- 日本に発生したエンドウ茎えそウイルスの性状
- 日本に発生したエンドウ茎えそウイルスの性状〔英文〕
- ニホン ニ ハッセイシタ エンドウ クキエソ ウイルス ノ セイジョウ エイブ
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Abstract
Pea stem necrosis virus (PSNV) was readily purified from infected pea plants by a chloroformbutanol procedure with yields of about 200mg/kg leaf tissues. Purified virus preparations contained a single sedimenting component with a sedimentation coefficient of 118S, buoyant density in cesium chloride of 1.35g/cm3 and nucleic acid content of 17%. Polyacrylamide gel electrophoresis demonstrated single nucleic acid component and single coat protein with estimated molecular weights of 1.53×106 and 38, 000 daltons, respectively. Nucleic acid extracted from the virus preparations was identified as single-stranded RNA by the following properties: positive orcinol reaction; susceptibility to RNase A both in 1×SSC or 0.1×SSC; increase of about 21% in maximum absorption and shift both the absorption maximum and minimum to higher wave-lengths by 4 nm on treatment with formaldehyde (1.8%). Preparations of purified particles of PSNV reacted with homologous antiserum (gel diffusion titer, 1:1, 024) but not with any antisera to 8 different plant viruses with isometric particles including tomato bushy stunt, galinsoga mosaic, carnation mottle or melon necrotic spot viruses. However, physicochemical properties were similar to those of carnation mottle and allied viruses.
Journal
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- Japanese Journal of Phytopathology
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Japanese Journal of Phytopathology 54 (2), 210-216, 1988
The Phytopathological Society of Japan
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Details 詳細情報について
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- CRID
- 1390282681380047104
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- NII Article ID
- 110002748086
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- NII Book ID
- AN0019269X
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- ISSN
- 18820484
- 00319473
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- NDL BIB ID
- 3197896
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed