Occurrence of strobilurin-resistant strains of Colletotrichum gloeosporioides (Glomerella cingulata), the causal fungus of strawberry anthracnose.

DOI JASI Web Site 11 Citations 17 References
  • INADA M.
    Saga Agricultural Research Center
  • ISHII H.
    National Institute for Agro-Environmental Sciences
  • CHUNG W. H.
    National Institute for Agro-Environmental Sciences National Chung Hsing University, Kuo Kuang Road
  • YAMADA T.
    Saga Agricultural Research Center
  • YAMAGUCHI J.
    Saga Agricultural Research Center
  • FURUTA A.
    Saga Agricultural Research Center

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  • ストロビルリン系薬剤耐性イチゴ炭疽病菌〔Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ　ｇｌｏｅｏｓｐｏｒｉｏｉｄｅｓ（Ｇｌｏｍｅｒｅｌｌａ　ｃｉｎｇｕｌａｔａ）〕の発生
  • ストロビルリンケイ ヤクザイ タイセイ イチゴ タンソビョウキン Colletotrichum gloeosporioides Glomerella cingulata ノ ハッセイ

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Abstract

Isolates of Colletotrichum gloeosporioides (Glomerella cingulata), obtained from strawberry leaves with anthracnose in Saga Prefecture in 2003, were bioassayed for sensitivity to strobilurin fungicides (azoxystrobin and kresoxim-methyl). The two isolates had little sensitivity to these fungicides, and they were regarded as resistant. Sequence analysis showed that the deduced amino acid at codon 143 of the mitochondrial cytochrome b gene was altered from glycine (GGT) to alanine (GCT). Polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) using the restriction enzyme ItaI could be employed in practice for rapid testing of resistance to strobilurin fungicides.<br>

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