Some Properties and Intracellular Appearance of Rice Waika Virus

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  • イネわい化ウイルスの性状ならびに細胞内所見
  • イネワイカ ウイルス ノ セイジョウ ナラビニ サイボウナイ ショケン エイブ

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Abstract

Rice waika virus (RWV) was purified by chloroform clarification and three cycles of differential centrifugations followed by sucrose density gradient centrifugation. RWV particles were spherical ones of about 30nm in diameter and showed an UV absorption spectrum characteristic of a nucleoprotein. The sedimentation coeffcient of the virus determined by analytical centrifugation was about 172S. Antiserum with the titer of 1/1, 024 for the agar gel diffusion test was prepared against RWV. By electron microscopy of thin sections, the presence of RWV particles was found to be restricted to phloem cells, in which two types of inclusion bodies and small vesicles were also observed. At the early stage of RWV infection, electron-dense crystalline inclusions showing latticed or tubular structures of protein nature developed, and the virus particles were produced first at their surface, and later within them. As the virus multiplication advanced, the crystalline inclusions changed to become electronlight granular inclusions, another type of inclusion body, which were easily found in RWV-infected cells and always associated with the virus particles. RWV particles gradually increased in number and accumulated as virus aggregates in the granular inclusions. The small vesicles developed around the inclusion bodies, and more often in the cytoplasm along and near the cell lumen. They contained fibrous materials like nucleic acid strands. Subsequently the virus particles were released from the inclusion bodies into the cytoplasm and vacuoles. From these observations the inclusion bodies were thought to be viroplasms. The virus particles in vacuoles often showed crystalline arrays. Phloem necrosis was very common, and accumulation of starch grains due to the phloem necrosis was often observed in chloroplasts in mesophyll cells.

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