Tomato Spotted Wilt Virus Isolates in Japan are Grouped into Two Strains.
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- TSUDA Shinya
- Plant Biotechnology Institute, Ibaraki Agricultural Center
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- HANADA Kaoru
- Kyushu National Agricultural Experiment Station
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- MINOBE Yuzo
- National Institute of Agrobiological Resources
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- KAMEYA-IWAKI Mitsuro
- Faculty of Agriculture, Yamaguchi University
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- TOMARU Keiichi
- NODAI Research Institute, Tokyo University of Agriculture
Bibliographic Information
- Other Title
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- トマト黄化えそウイルスの本邦分離株は2系統に分けられる
- トマト黄化えそウイルスの本邦分離株は2系統に分けられる〔英文〕
- トマト オウカエソ ウイルス ノ ホンポウ ブンリカブ ワ 2 ケイトウ ニ
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Abstract
Five isolates of tomato spotted wilt virus (TSWV), designated N, M, P, W and K, collected from different prefectures in Japan, were characterized and compared on their properties of nucleocapsid (N) protein and S RNA. Nucleocapsid of each isolate was purified by ultracentrifugation followed by metrizamide isopycnic density-gradient. Molecular weights of N proteins were 30 K in case of N, M and P isolates, while those of W and K isolates were 32 K. Moreover, difference of chromatograms between 30 K and 32 K N proteins digested by lysyl endpeptidase was observed. By Western blot analysis, specific antibody to N protein of N isolate reacted to N protein of N, M and P isolates, whereas specific antibody to that of W isolate could detect only those of W and K isolates without non-specific reactions. When S RNA was compared by glyoxalated agarose gel electrophoresis, molecular weights of S RNAs of N, M and P isolates were 1.02×106, while those of W and K isolates were 1.21×106. In Northern blot hybridization analysis using S RNA from either N or W isolate as probe, S RNA from N isolate tightly hybridized with S RNAs of N, M and P isolates, and S RNA from W isolate hybridized only with those of W and K isolates. Based on the results obtained, TSWV isolates which occurred in Japan were grouped into two strains. The one was designated as ordinary strain (TSWV-O; N, M and P isolates) and the other was watermelon strain (TSWV-W; W and K isolates).
Journal
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- Japanese Journal of Phytopathology
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Japanese Journal of Phytopathology 59 (6), 626-634, 1993
The Phytopathological Society of Japan
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Details 詳細情報について
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- CRID
- 1390282681380826112
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- NII Article ID
- 110002736646
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- NII Book ID
- AN0019269X
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- ISSN
- 18820484
- 00319473
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- NDL BIB ID
- 3869206
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed