Simple Method for Simultaneous Determination of Chlorophyll and Carotenoids in Tomato Fruit.

DOI 25 Citations
  • NAGATA Masayasu
    National Research Institute of Vegetables, Ornamental Plants and Tea, Ministry of Agriculture, Forestry and Fisheries
  • YAMASHITA Ichiji
    National Research Institute of Vegetables, Ornamental Plants and Tea, Ministry of Agriculture, Forestry and Fisheries

Bibliographic Information

Other Title
  • トマト果実に含まれるクロロフィルおよびカロテノイドの同時，簡便定量法

Description

The contents of chlorophyll and carotenoid in tomato fruits have been analyzed respectively by different methods in a conventional way. We studied simple method for simultaneous determination of the pigments in tomato. All pigments in sample were extracted with acetone-hexane (4:6) at once, then optical density of the supernatant were measured at 663nm, 645nm, 505nm and 453 nm by spectrophotometer at the same time. From these values, the content of chlorophyll a, b, lycopene and β-carotene could be estimated using our proposed equations. Tomatoes of different ripening stage were analyzed by our method. Also the same samples were analyzed by conventional methods. The results by our method were similar to those of chlorophyll contents by MACKINNEY'S method and of lycopene contents by KIMURA'S method. It confirmed the availability of our method.

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Details 詳細情報について

  • CRID
    1390282681381265024
  • NII Article ID
    130003788960
  • DOI
    10.3136/nskkk1962.39.925
  • COI
    1:CAS:528:DyaK3sXhvFShsrc%3D
  • ISSN
    00290394
    http://id.crossref.org/issn/00290394
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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