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- KAWASHIMA KOJI
- National Food Research Institute, Ministry of Agricalture and Forestry.
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- Umeda KEIJI
- National Food Research Institute, Ministry of Agricalture and Forestry.
Bibliographic Information
- Other Title
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- 固定化酵素の製造 V 放射線共重合法により製造した固定化インベルターゼの諸性質(2)
- コテイカ コウソ ノ セイゾウ ニカンスルケンキュウ 5 ホウシャセン キョウ
- (第5報)放射線共重合法により製造した固定化インベルターゼの諸性質について(その2)
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Abstract
The mixture of water soluble synthetic monomers and invertase solution was frozen in dry ice/acetone and radiocopolymerized by gamma-ray. The characteristics of resulting polymer which was the membranous immobilized invertase were investigated succeeding to the former report.<BR>Following results were obtained.<BR>1) There was no significant difference in the Km value between native and immobilized invertase.<BR>2) A decrease in activity of immobilized invertase was accompained by an increase in enzyme concentration and it was 8.5 to 91.5%.<BR>3) Immobilized invertase showed good stability in a dry state even if it was stored at 35°C. The residual activity was about 74 to 95% after 60 days storage and about 80% after 130 days storage.<BR>4) After the repeating usage for 30 times, immobilized enzyme showed about 70% of activity comparing to the original preparation.<BR>5) Invertase became more stable to aniline and urea when immobilized but it became more labile to ethanol, guanidin, Hg and Cu.
Journal
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- NIPPON SHOKUHIN KOGYO GAKKAISHI
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NIPPON SHOKUHIN KOGYO GAKKAISHI 23 (5), 206-210, 1976
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390282681381620992
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- NII Article ID
- 130003966774
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- NII Book ID
- AN00192587
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- NDL BIB ID
- 1712436
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- ISSN
- 00290394
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed