The Condition of Oligosaccharides Formation by .BETA.-Galactosidase from Alternaria alternate.

DOI

Bibliographic Information

Other Title
  • Ａｌｔｅｒｎａｒｉａ　ａｌｔｅｒｎａｔｅ　β‐ガラクトシダーゼのオリゴ糖生成条件

Abstract

The conditions of lactose hydrolysis and oligosaccharides formation of purified β- galactosidase from Alternaria alternate was studied. As the results of HPLC and enzymatic analysis, the β-galactosidase formed 6 kinds of oligosaccharides. It was assumed that 5 kinds were disaccharides and one kind was trisaccharide. There was the high posifive correlation between amount of liberated glucose and oligosaccharides. Formation of oligosaccharides was accelerated in high substrate concentration and high reaction temperature. Amount of oligosaccharides formed in lactose hydrolysis reached maximum at the optimum pH, 5.0-5.5. The rate of oligosaccharide formation on alkaline side was higher than onacdic side, and the kinds of oligosaccharides were different in different reaction pH.

Journal

Details 詳細情報について

  • CRID
    1390282681381713920
  • NII Article ID
    130003968119
  • DOI
    10.3136/nskkk1962.40.5_362
  • ISSN
    00290394
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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