Determination of L-Ascorbic acid, tocopherol, carotene and chlorophyll in various teas.

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Other Title
  • 各種茶に含まれるＬ‐アスコルビン酸，トコフェロール，カロチン，クロロフィルについて

Abstract

L-Ascorbic aid, tocopherol, carotene and chlorophyll contents of various teas were determined by high performance liquid chromatography. Ascorbic acid was measured on Inertsil ODS-2 with amperometer at+500mV vs Ag/AgC1. Tocopherols were extracted from the sample with hexane and pretreated using florisil mini column, and determined on LiChrosorb NH2 With a flouorescence detection. Carotene and chlorophylls were separated on Zorbax ODS and detected with visible wavelength detector. All kinds of those compounds examined were found much more in sencha than semi-fermented (Oolong) and fermented (Black) teas. The average contents of ascorbic acid, a-tocopherol, a and β-carotene in sencha were 167, 13.5, 3.0 and 40.2mg/100g, respectively. In sencha, carotenes(α+β; X) were correlated With chlorophylls (a+b; Y): Y= 6.73 X-86.5, r=0.912. Those compounds in the solutions decocted from teas by usual way were also determined. These compounds except ascorbic acid did not detected.

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Details 詳細情報について

  • CRID
    1390282681382966528
  • NII Article ID
    130003788782
  • DOI
    10.3136/nskkk1962.36.11_927
  • COI
    1:CAS:528:DyaK3cXnsFCgsw%3D%3D
  • ISSN
    00290394
    http://id.crossref.org/issn/00290394
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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