アンの崩壊粒子及び損傷粒子の定量法　Ｉ　　アン粒子のアミラーゼ処理による澱粉の溶解

DOI Web Site

書誌事項

タイトル別名
  • An enzymatic method for determination of ruptured and damaged granules in Ann samples. Part I. Solubilization of starch by treatment of Ann granules with .ALPHA.-amylase.
  • アン リュウシ ノ アミラーゼ ショリ ニ ヨル デンプン ノ ヨウカイ
  • An Enzymatic Method for Determination of Ruptured and Damaged Granules in Ann Samples Part I
  • アンの崩壊粒子及び損傷粒子の定量法(第1報)

この論文をさがす

抄録

Dried plain Adzuki Ann granules prepared from Adzuki bean (Vigna angularis) and samples prepared by grinding an aqueous suspension of the dried Adzuki Ann granules by a homogenizer were used as models of intact and ruptured Ann granules respectively. This paper describes a method to determine the proportion of ruptured granules in a standard Ann sample containing intact and ruptured granules in various ratios, by measuring the percentage of starch solubilized by α-amylase. The dried sample (24mg) suspended in a buffer (pH 5.9) was allowed to react with α-amylase (1 unit) at 35°C for 30min. The suspension was then placed in a boiling water bath for 10min, cooled and centrifuged, and the amount of starch solubilized was measured. The amount of starch solubilized by the treatment with HCl (0.25N) instead of α-amylase was regarded as the total amount of starch in the sample. The percentage of starch solubilized by the α-amylase treatment was defined as the ratio of the amount of starch solubilized by the α-amylase treatment to that by the HCl treatment. It was found that the percentage of starch solubilized by the α-amylase treatment was about 2% for the intact granules and about 98% for the ruptured granules and increased linearly with a increasing proportion of ruptured granules in the standard Ann sample. The results suggest that the percentage of starch solubilized by the α-amylase treatment might be applied to the determination of ruptured and damaged granules in Ann samples.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ