Rapid and Simple Detection of Coliform Bacteria in Milk Using Menadione-Catalyzed Luminol Chemiluminescence Assay

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  • 化学発光法による牛乳中大腸菌群の簡易迅速検出
  • カガク ハッコウホウ ニ ヨル ギュウニュウチュウ ダイチョウキングン ノ カンイ ジンソク ケンシュツ

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Menadione-catalyzed luminol chemiluminescence assay was evaluated for rapid detection of coliform bacteria in milk. The assay was evaluated using raw milk (non-pasteurized) samples and pasteurized milk samples spiked with Escherichia coli, Enterobacter aerogenes, Klebsiella oxytoca, Citrobacter freundii. The detection limit in this assay was 105-104CFU/ml using a 96-well plate with BGLB broth. Our study revealed that coliform contamination of 101-100CFU/ml of milk could be detected after 18 h of incubation at 35°C in BGLB medium. Moreover, this assay detected gas production in samples of BGLB after only 18h of a 48-h incubation. As this assay is convenient, quick and inexpensive compared to the conventional method, it should be useful for the rapid detection of coliform contamination in pasteurized milk.

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