Separation of Collagen from Fish Scales by Treatment of Dilute Hydrochloric Acid Aqueous Solution.

DOI Web Site 3 Citations 16 References
  • Takenaka Atsushi
    Department of Materials Science, Yonago National College of Technology
  • Okada Akira
    Department of Materials Science, Yonago National College of Technology
  • Iwai Kenta
    Department of Materials Science, Yonago National College of Technology
  • Ayaki Yoshikazu
    Department of Materials Science, Yonago National College of Technology

Bibliographic Information

Other Title
  • 希塩酸処理による魚鱗からのコラーゲンの分離
  • キエンサン ショリ ニ ヨル ギョリン カラ ノ コラーゲン ノ ブンリ

Search this article

Abstract

Fish scales are mainly composed of collagen and hydroxyapatite (HAp). Since HAp is solubilized in a dil. hydrochloric acid aq. soln., crude collagen could be separated from carp (Cyprinus carpio Linnaeus) scales by a HCl treatment. The optimal conditions for the HCl treatment were examined and compared to those applied for sardine (Sardinops melanostictus Temminck et Schlegel) scales, reported previously. The utilization of the separated collagen is discussed on the basis of the contents of heavy metals in the ash obtained by asking of the scales at 1000°C. The determination of hydroxyproline, which is a kind of characteristic amino acid for collagen, and SDS-PAGE analysis show that the crude collagen obtained is Type-I collagen.

Journal

Citations (3)*help

See more

References(16)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top