Mechanism of Apoptosis Induced by Polyterpene from Buna-shimeji (Hypsizigus marmoreus) in HL-60 Cells
-
- Mizumoto Hiroko
- United Graduate School of Agricultural Sciences, Kagoshima University
-
- Ohnogi Hiromu
- Biotechnology Research Laboratories, Takara Bio, Inc.
-
- Mizutani Shigetoshi
- Biotechnology Research Laboratories, Takara Bio, Inc.
-
- Enoki Tatsuji
- Biotechnology Research Laboratories, Takara Bio, Inc.
-
- Asada Kiyozo
- Biotechnology Research Laboratories, Takara Bio, Inc.
-
- Sugimoto Yasushi
- United Graduate School of Agricultural Sciences, Kagoshima University
-
- Kato Ikunoshin
- Biotechnology Research Laboratories, Takara Bio, Inc.
Bibliographic Information
- Other Title
-
- ブナシメジ由来ポリテルペンのHL‐60白血病細胞におけるアポトーシス誘導機構
- ブナシメジ由来ポリテルペンのHL-60白血病細胞におけるアポトーシス誘導機構
- ブナシメジ ユライ ポリテルペン ノ HL 60 ハッケツビョウ サイボウ ニ オケル アポトーシス ユウドウ キコウ
Search this article
Abstract
Hypsiziprenol A9 is a polyterpene isolated from the fruiting body of the Japanese edible mushroom Buna-shimeji (Hypsizigus marmoreus). Although our recent studies revealed that hypsiziprenol A9 has strong anti-tumor activity against tumor-bearing mice, its mechanism of action remains unclear. To elucidate further its anti-tumor action, we examined in detail hypsiziprenol A9-induced apoptosis in human cancer cell lines.<BR>We observed that hypsiziprenol A9 strongly inhibited the growth of human promyelocytic leukemia HL-60 cells and several other cancer cell lines in a dose-dependent manner. Hoechst 33342 staining showed DNA fragmentation and condensation of chromatin in HL-60 cells treated with hypsiziprenol A9. DNA laddering, a hallmark of apoptosis, was detected using agarose gel electrophoresis. In addition, flow cytometric analysis confirmed that hypsiziprenol A9 increased sub-G1/G0 populations in a time-dependent manner. Hypsiziprenol A9 also increased activities of caspase-2, -3, -8 and -9 in a dose-dependent manner in the apoptotic HL-60 cell. Furthermore, JC-1 fluorescent staining determined that hypsiziprenol A9-induced apoptosis was associated with a loss of mitochondrial membrane potential.<BR>These results suggest that hypsiziprenol A9 inhibits the growth of HL-60 cells by inducing apoptosis that is mediated through mitochondrial membrane potential loss and caspase activation.
Journal
-
- Nippon Shokuhin Kagaku Kogaku Kaishi
-
Nippon Shokuhin Kagaku Kogaku Kaishi 55 (12), 612-618, 2008
Japanese Society for Food Science and Technology
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681385530112
-
- NII Article ID
- 10024854738
-
- NII Book ID
- AN10467499
-
- ISSN
- 18816681
- 1341027X
-
- NDL BIB ID
- 9735925
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed