Some Phylogenetical Aspects on the Occurrence of Somatostatin in the Gastro-Entero-Pancreatic Endocrine System
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- FALKMER Sture
- Department of Pathology, University of Umeå
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- ELDE Robert P.
- Department of Anatomy, University of Minnesota School of Medicine
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- HELLERSTRÖM Claes
- Department of Histology, University of Uppsala
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- PETERSSON Birger
- Department of Histology, University of Uppsala
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- EFENDIC Suad
- Department of Endocrinology, Karolinska Hospital
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- FOHLMAN Jan
- Department of Biochemistry, University of Uppsala
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- SILJEVALL Jan-Bertil
- the Kristineberg Marine Biological Station
書誌事項
- タイトル別名
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- A Histological and Immunocytochemical Study, Combined with Quantitative Radioimmunological Assays of Tissue Extracts
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説明
Rodioimmunoassayable somatostatin (SRIF) was found in acid ethanol extracts from various parts of the gastro-entero-pancreatic (GEP) endocrine system in reptiles, amphibians, teleost bony fish, cartilaginous fish, and jawless fish, as well as in a deuterostomian invertebrate, the tunicate, Ciona intestinalis. The cellular sites could, as a rule, be easily visualized light-microscopically by the peroxidase-anti-peroxidase (PAP) immunocytochemical procedure, using guinea-pig and rabbit antisera against synthetic SRIF. The standard Hellerström-Hellman technique, used to detect argyrophil SRIF-storing D cells, failed to visualize the SRIF cells in the GEP endocrine system of the tunicate and of the jaw-less fish. Moreover, the results confirmed the previous description that this technique only exceptionally (and sometimes only after further modifications) gave positive results when applied to the GEP endocrine system of bony fish, amphibians, and reptiles. In cartilaginous fish, however, it worked adequately and confirmed the radioimmunological and immunocytochemical observations.<br>In the mucosa of the alimentary tract and in the parenchyma of its associated glands of one echinoderm and two pelecypod molluscs and one crustacean arthropod no signs of the occurrence of SRIF-storing cells were observed using the three correlated procedures. In several of these tissues, signs of the occurrence of insulin-producing cells had previously been observed. Thus, SRIF seems to appear at a later evolutionary stage than insulin.<br>The principal islets (Brockmann corpusles) of the marine teleost fish, Cottus scorpius, had the highest concentrations of radioimmunoassayable SRIF of all the GEP organs and tissues investigated, viz. about 200ng/mg wet weight. Nevertheless, it was only 1/5 of the actual insulin content.
収録刊行物
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- Ａｒｃｈｉｖｕｍ ｈｉｓｔｏｌｏｇｉｃｕｍ ｊａｐｏｎｉｃｕｍ
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Ａｒｃｈｉｖｕｍ ｈｉｓｔｏｌｏｇｉｃｕｍ ｊａｐｏｎｉｃｕｍ 40 (Supplement), 99-117, 1977
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