Comparative Analyses of Proteoglycan-containing Powder Prepared from Salmon Nasal Cartilage and Extracted with Various Solvents

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  • Miura Ayako
    Laboratory of Food Science, Faculty of Education, Hirosaki University
  • Ito Seiko
    Laboratory of Food Science, Faculty of Education, Hirosaki University
  • Kato Yoji
    Laboratory of Food Science, Faculty of Education, Hirosaki University

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  • サケ鼻軟骨粉末から異なる溶媒で抽出したプロテオグリカンの性質の比較
  • サガハナ ナンコツ フンマツ カラ コトナル ヨウバイ デ チュウシュツ シタ プロテオグリカン ノ セイシツ ノ ヒカク

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Proteoglycan (PG)-containing powder was prepared from salmon nasal cartilage and extracted with the following solutions : distilled water ; 4 M guanidine hydrochloride ; 7 M urea ; 0.5 M sodium hydroxide ; 4 % acetic acid ; 1 % malic acid ; 1 % citric acid ; and 1 % ascorbic acid. Comparisons of PG between the solvent types revealed varying amounts of uronic acid and protein. DEAE Sephacel ion exchange chromatography showed that the PG elution patterns of all extracts were similar to those of PG prepared by the conventional 4 % acetic acid extraction method, thus demonstrating their similar electrostatic properties. Sepharose CL-4B gel filtration chromatography was then used to investigate molecular weight distribution, and the rate of PG distribution in the high molecular weight fraction was shown to be higher in the water extracts than in both the acid and alkaline extracts. These results suggest that hig-molecular-weight PG is the natural form of PG.

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