Validation of Method for Determining <i>O</i>-methylated Catechin in ‘Benifuuki’ Green Tea (<i>Camellia sinensis </i>L.) by Interlaboratory Study

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  • Homura Yuji
    Food and Agricultural Materials Inspection Center
  • Hiejima Yusuke
    Food and Agricultural Materials Inspection Center
  • Kodama Takashi
    Food and Agricultural Materials Inspection Center
  • Tanaka Masumi
    Food and Agricultural Materials Inspection Center
  • Horie Hideki
    NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
  • Suzuki Tadanao
    Food and Agricultural Materials Inspection Center National Food Research Institute, National Agriculture and Food Research Organization
  • Yasui Akemi
    National Food Research Institute, National Agriculture and Food Research Organization

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Other Title
  • べにふうき緑茶中のメチル化カテキン測定法の室間共同試験による妥当性確認
  • ベ ニ フウキ リョクチャ チュウ ノ メチルカ カテキン ソクテイホウ ノ シツカン キョウドウ シケン ニ ヨル ダトウセイ カクニン

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<p>An analytical method for the determination of O-methylated catechin in ‘benifuuki’ green tea (Camellia sinensis L.) was investigated and evaluated by an interlaboratory study. Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me) was well separated within ten minutes by high performance liquid chromatography (HPLC). The detection limit (S/N=3) of EGCG3"Me in this method was 0.005% (w/w). Ten participating laboratories independently analyzed five test materials (five pairs of blind duplicates) in an interlaboratory study. The repeatability (RSDr) and reproducibility (RSDR) of the evaluated methods were 1.4∼2.0% and 1.6∼5.7%, respectively, in a concentration range from 1.1 to 1.9% (w/w). The HorRat values of the EGCG3"Me concentration were 0.39 to 1.4. The present study showed that this method has acceptable precision.</p>

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