Solubilization of Tocopherols by Using Cyclization Reaction with Cyclodextrin Glucanotransferase

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  • サイクロデキストリングルカノトランスフェラーゼの環化反応を利用したトコフェロールの可溶化
  • サイクロデキストリン グルカノトランスフェラーゼ ノ カンカ ハンノウ オ リヨウ シタ トコフェロール ノ カヨウカ

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Vitamin E (VE : tocopherols and tocotrienols) is well known to have an anti-oxidative ability and the potentiality to prevent lifestyle-related diseases through its antioxidant function. In this paper, α-tocopherol (α-TOC), which was trimethyl type and had the most strong biological effect among tocopherols and tocotrienols, and δ-tocopherol (δ-TOC), which was monomethyl type, were applied to prepare complexes with cyclodextrin (CD) by cyclization reaction with cyclodextrin glucanotransferase.<br>In the newly developed inclusion method, β-CD was the most suitable for both α-TOC and δ-TOC to form inclusion complexes. α-CD formed soluble complexes with α-TOC and δ-TOC without ethanol, whereas γ-CD formed insoluble complexes with δ-TOC independent of ethanol concentration.<br>Soluble starch solubilized a small amount of α-TOC and δ-TOC without ethanol, while α-TOC and δ-TOC formed insoluble complexes in 30% ethanol solution. Cyclization reaction with cyclodextrin glucanotransferase exhibited superiority to produce soluble complexes with α-TOC than the conventional method.

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