High Performance Liquid Chromatography Quantification of Total Astaxanthin in Health Foods

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  • 健康食品中の総アスタキサンチンのＨＰＬＣ定量法
  • ケンコウ ショクヒン チュウ ノ ソウ アスタキサンチン ノ HPLC テイリョウホウ

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Abstract

Astaxanthin is a red carotenoid pigment mostly distributed among marine organisms. Due to the multifunctional physiological benefits through its potent antioxidant activity, many products are supplemented with astaxanthin in the current market place. Although the demands for quantitative verification of astaxanthin have rapidly increased, the existence of its esterified forms in the products sometimes hampers accurate determination. In this study, high performance liquid chromatography was applied to examine their versatile quantification after enzyme-hydrolysis of esterified astaxanthin from Haematococcus sp. The activity among nine enzymes was compared using the chloroform extract of Haematococcus sp. A cholesterol esterase from Pseudomonas fluorescens gave the maximum activity at the optimized reaction condition of 37°C for 120min. In the validation study, an astaxanthin ester solution was added to three health food products to obtain an average recovery with a range of 97-101% and the residual standard deviation of<2% in the concentration range of 1.7-800mg/100g sample. A repeatability test using health food tablets containing Haematococcus extract was repeated seven times to determine precision of the method, giving a residual standard deviation of<2%. These results suggest that the proposed method was useful for analyzing total astaxanthin content in food supplemented with esterified astaxanthin from Haematococcus sp.

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