書誌事項
- タイトル別名
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- The Liquefaction Effects of Crude-Actinidin Treatments with Heating on Adult Cattle Aponeuroses Collagen under acidic conditions
- サンセイ ジョウケン ニ オケル ギュウケンマク コラーゲン ノ ヨウカイ ニ オヨボス ソアクチニダイン ト カネツ ノ ヘイヨウ コウカ
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説明
About 1% of cattle aponeuroses was extracted after 60min of heating in hot water at 85°C. The collagen subunit chains containing β- and α-chains were not detected in the insoluble fractions of cattle aponeuroses after 60min heating at 80°C by SDS-PAGE analysis. The insoluble fractions were treated with plant thiol proteases such as crude-actinidin, papain, bromelain and ficin at 70°C for 60min under acid conditions, and then the effects of extraction from the treated-cattle aponeuroses were analyzed by measuring the yield of total and extracted-collagen. The percentage of extraction from the cattle aponeuroses treated with crude-actinidin, papain, bromelain or ficin was at a level of 34.7, 11.7, 6.6, and 2.8% yield, respectively (p<0.01). Furthermore, collagen subunit chains were detected in the heated-aponeuroses (85°C, 60min) treated with crude-actinidin at 37°C for 3h in 0.5M acetic acid, but not detected in the un-heated aponeuroses extracted with 0.5M acetic acid at 37°C for 3h.
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 50 (11), 506-510, 2003
公益社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282681387010688
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- NII論文ID
- 10012529361
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- NII書誌ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
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- NDL書誌ID
- 6755068
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可