酸性条件における牛けん膜コラーゲンの溶解に及ぼす粗アクチニダインと加熱の併用効果

書誌事項

タイトル別名
  • The Liquefaction Effects of Crude-Actinidin Treatments with Heating on Adult Cattle Aponeuroses Collagen under acidic conditions
  • サンセイ ジョウケン ニ オケル ギュウケンマク コラーゲン ノ ヨウカイ ニ オヨボス ソアクチニダイン ト カネツ ノ ヘイヨウ コウカ

この論文をさがす

説明

About 1% of cattle aponeuroses was extracted after 60min of heating in hot water at 85°C. The collagen subunit chains containing β- and α-chains were not detected in the insoluble fractions of cattle aponeuroses after 60min heating at 80°C by SDS-PAGE analysis. The insoluble fractions were treated with plant thiol proteases such as crude-actinidin, papain, bromelain and ficin at 70°C for 60min under acid conditions, and then the effects of extraction from the treated-cattle aponeuroses were analyzed by measuring the yield of total and extracted-collagen. The percentage of extraction from the cattle aponeuroses treated with crude-actinidin, papain, bromelain or ficin was at a level of 34.7, 11.7, 6.6, and 2.8% yield, respectively (p<0.01). Furthermore, collagen subunit chains were detected in the heated-aponeuroses (85°C, 60min) treated with crude-actinidin at 37°C for 3h in 0.5M acetic acid, but not detected in the un-heated aponeuroses extracted with 0.5M acetic acid at 37°C for 3h.

収録刊行物

参考文献 (15)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ