コイ卵におけるカルパインおよびカルパスタチンの存在

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タイトル別名
  • Detection of calpain and calpastatin in carp eggs.
  • コイ卵におけるカルパインおよびカルパスタチンの存在〔英文〕
  • コイ ラン ニ オケル カルパイン オヨビ カルパスタチン ノ ソンザイ エイ

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抄録

In order to examine the participation of calpain and calpastatin in the biological process, carp eggs were chosen as the experimental material. Calpain activity was hardly detected in carp egg crude extract. However, the activities of calpain and calpain inhibitor became recognizable through the DEAE-cellulose chromatography of the crude extract. Carp egg calpain partially purified by further column chromatographies was classified as calpain II from the degree of Ca2+ requirement for the activity. The optimum pH was 7.1-7.2 and the activity was strongly inhibited by leupeptin, E-64 and antipain.<br>Carp eggs seemed to contain also calpastatin, endogenous inhibitor for calpain, since the inhibitory activity was extremely heat stable.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 51 (8), 1281-1286, 1985

    公益社団法人 日本水産学会

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