Electrophoretic Identification of Three Subspecies of the Genus <i>Lagocephalus</i> Pufferfish

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  • HASHIMOTO Kanehisa
    Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo
  • WATABE Shugo
    Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo
  • NAKAGAWA Takayuki
    Laboratory of Biochemistry, Tokyo University of Fisheries
  • SORITA Kenji
    Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo

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  • サバフグ属３亜種の電気泳動的鑑別
  • サバフグゾク 3 アシュ ノ デンキ エイドウテキ カンベツ

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Abstract

Attempts were made to identify electrophoretically three subspecies of the genus Lagocephalus pufferfish: “kurosabafugu” L. lunaris subsp., “shirosabafugu” L. lunsris spadiceus, and “dokusabafugu” L. lunaris lunaris.<br> The extracts were prepared from the ordinary muscle of those three subspecies, and analyzed for patterns of sarcoplasmic protein and several enzymes by polyacerylamide gel electrophoresis.<br> The three subspecies clearly differed from each other in sarcoplasmic protein pattern, though small individual variations were recognized in L. lunaris spadiceus and L. lunaris lunaris.<br> Of the exzymes tested, the most specific to the subspecies was malate dehydrogenase (MDH), followed by lactate dehydrogenase (LDH) and phosphoglucomutase (PGM).

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