The Structure of an Extracellular Polysaccharide from a Marine Strain of Enterobacter.

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  • 海洋性<i>Enterobacter</i>の生産する菌体外多糖の構造
  • 海洋性Enterobacterの生産する菌体外多糖の構造〔英文〕
  • カイヨウセイ Enterobacter ノ セイサンスル キンタイガイ タトウ

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Abstract

An extracellular, acidic polysaccharide was isolated from a marine strain of Enterobacter sp., designed MU-3, which was tentatively identified as E. agglomerans. The structure of this polysaccharide was determined by techniques of methylation, mild acid hydrolysis, periodate oxidation, Smith hydrolysis, and β-elimination. 1H and 13C NMR spectroscopy was used to establish the nature of the anomeric linkages of the polysaccharide and of oligosaccharides derived through degradative procedures. The polysaccharide has a branched structure containing a backbone chain of →3)-β-D-Galp (1→3)-α-D-Galp (1→6)-β-D-Glcp (1→, and a side chain of α-D-Galp (1→4)-β-D-GlcUAp (l→. The glucuronic acid residue in the side chain joins through C-4, to β-D-galactose residue in the main chain. The terminal galactose in the side chain bears a pyruvic acetal substituent at positions C-4 and C-6.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 57 (10), 1949-1956, 1991

    The Japanese Society of Fisheries Science

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