Re-examination of protective effect of ATP on thermal inactivation of myosin Ca-ATPase.

DOI JASI Web Site 5 Citations
  • Yoshioka Takeya
    Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University Central Research Laboratory, Nippon Suisan Co., Ltd.
  • Hamai Masashi
    Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University Central Research Laboratory, Nippon Suisan Co., Ltd.
  • Konno Kunihiko
    Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University
  • Arai Ken-ichi
    Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University

Bibliographic Information

Other Title
  • ＡＴＰによる魚類ミオシンの熱変性抑制の再検討
  • ATP ニ ヨル ギョルイ ミオシン ノ ネツ ヘンセイ ヨクセイ ノ サイケ

Search this article

Description

The mechanism involved in protective effect of ATP on thermal inactivation of myosin Ca-ATPase was re-examined. 1) ATP concentration required for full protection against thermal denaturation is myosin concentration dependent, and two concentrations were similar to each other. 2) The protection by ATP is more effective in the presence of Mg2+ than in the presence of Ca2+ or EDTA, suggesting the high affinity of ATP-Mg to the ATPase active site. 3) The protective effects of triphosphate compounds (ATP and AMPPNP) were distinguish-able from those of diphosphate compounds (ADP, PPi) in their concentration dependence and in the maximal protection achieved, and monophosphate compounds (AMP and IMP) were unable to protect myosin from inactivation at all.

Journal

Citations (5)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top