A rapid method to determine microsomal metabolism of organophosphate pesticides.

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  • MCLEAN Scott
    Laboratory of Marine Biochemistry, Faculty of Fiseries, Kagoshima University
  • SAMESHIMA Muneo
    Laboratory of Marine Biochemistry, Faculty of Fiseries, Kagoshima University
  • KATAYAMA Teruhisa
    Laboratory of Marine Biochemistry, Faculty of Fiseries, Kagoshima University
  • IWATA Jiro
    Kagoshima Prefecture Fisheries Experimentsl Station
  • OLNEY Charles E.
    Department of Food Science and Technology, Nutrition and Dietetics, University of Rhode Island
  • SIMPSON Kenneth L.
    Department of Food Science and Technology, Nutrition and Dietetics, University of Rhode Island

Bibliographic Information

Other Title
  • ミクロソームにおける有機リン殺虫剤代謝の迅速検出法
  • ミクロソームにおける有機リン殺虫剤代謝の迅速検出法〔英文〕
  • ミクロソーム ニ オケル ユウキ リン サッチュウザイ タイシャ ノ ジンソク

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Description

This research was undertaken to determine if a rapid and accurate procedure incorporating gas-liquid chromatography could be developed to measure microsomal metabolism of organophos-phate pesticides, i. e. malathion, diazinon, and fenitrothion, in the lives of fish.<br> Three fish species important to the aquaculture industry of japan, Sarother odon niloticus, Cyprinus carpio, and Seriola quingueradita, were used to provide microsomal preparations in order to observe specific differences in xenobiotic metabolism.<br> Each assay mixture contained pesticide and bovine serum albumin in a phosphate buffer solution and raw or boiled microsomal preparations. These mixtures were tested fro metabolism of pesticide in the presence or absence of NADPH.<br> The reaction was allowed to proceed at 30°C for a specific amount of time dependent on the particular fish/ pesticide combination. Unreacted pesticide was extracted and was detected by gasliquid chromatography with a flame-hpotometric detector.<br> Two different metabolic reactions were detected in the microsomal preparations of the fish tested. Malathion was metabolized by a NADPH independent enzyme system, while diazinon and fenitro-thion were metabolized by a NADPH dependent enzyme system.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 50 (8), 1419-1423, 1984

    The Japanese Society of Fisheries Science

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