Separation and Identification of Antioxidant Peptides from Proteolytic Digest of Dried Bonito.

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  • かつお節タンパク質のプロテアーゼ消化物からの抗酸化ペプチドの分離と同定
  • カツオブシ タンパクシツ ノ プロテアーゼ ショウカブツ カラ ノ コウサンカ ペプチド ノ ブンリ ト ドウテイ

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A peptidic fraction having antioxidant activity was separated from the proteolytic digest of dried bonito protein by ion-exchange chromatography and gel filtration. Further separation by ODS HPLC afforded eleven active peptides; Val-Lys-Leu, Val-Val-Lys-Leu, Val-Lys-Val, Pro-Lys-Ala-Val, Ile-Lys-Leu, Val-Pro-Ser-Gly-Lys, Glu-Ala-Lys, Phe-Val-Ala-Gly-Lys, Lys-Ala-Ile, Lys-Val-Ile, and Lys-Asp. The structure-activity relationship of these peptides was discussed.

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