サケ科魚類せっそう病原因菌Ａｅｒｏｍｏｎａｓ　ｓａｌｍｏｎｉｃｉｄａの産生する病因物質について　ＩＩＩ　　Ａｅｒｏｍｏｎａｓ　ｓａｌｍｏｎｉｃｉｄａ　Ａｒ‐４　（ＥＦＬＤ）の産生するプロテアーゼの特性

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タイトル別名
  • Enzymatic properties of the purified extracellular protease of <i>Aeromonas salmonicida</i>, Ar-4 (EFDL)
  • Aeromonas salmonicida,Ar 4 EFDL ノ サンセイス

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説明

In a previous paper, we examined the toxicity of the extracellular protease of A. salmonicida, Ar-4 (EFDL) on yamabe (Oncorhynchus masou f. ishikawai) and goldfish (Carassius auratus). From these results, we considered the protease secreted by this bacterium was a causative agent of furunculosis.<br> In this paper, we observed enzymatic properties of the purified protease. The results obtained were summarized as follows:<br> 1. The molecular weight of purified protease was estimated to be 71, 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis.<br> 2. The purified protease showed maximal activity at pH 9.4 and 50°C. It was stable over the pH range 5.0 to 10.0 and was completely inactivated by temperature at 56°C.<br> 3. The protease was presumably classified an alkaline serine protease and it showed chymotryptic properties since it was significantly inhibited by diisopropylfluorophosphate (DFP) and tosyl-phenylalanine-chloromethyl ketone (TPCK) and hydrolyzed N-benzoyl-L-tyrosine ethyl ester (BTEE).

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 50 (1), 145-150, 1984

    公益社団法人 日本水産学会

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