RAPD-PCRおよびアイソザイムパターンによるギンブナ一腹子のクローン性の証明
書誌事項
- タイトル別名
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- Clonal Nature of Offspings of Ginbuna Carassius langsdorfii by RAPD-PCR and Isozyme Patterns.
- ＲＡＰＤ‐ＰＣＲおよびアイソザイムパターンによるギンブナー腹子のクローン性の証明
- RAPD-PCR オヨビ アイソザイム パターン ニヨル ギンブナ イチ ハラ
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The eggs of Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperm of a Gengoroubuna Carassius cuvieri and a commom carp Cyprinus carpio respectively. DNA extracted from blood of parents and offsprings were amplified using 20 random primers and separated by electrophoresis (RAPD-PCR). Isozymes of muscle and liver of parents and offsprings were assayed by the horizontal starch gel electrophoresis. Distinct differences by maternal fish were observed in the patterns of both RAPD-PCR (17 primers) and isozymes (CK, EST, GPI, PGM), but those of offsprings produced by crossing with Gengoroubuna and common carp respectively were the same completely within the maternal fish. Therefore, the results obtained in this study verified that the offsprings from Ginbuna were clonal genetically, being the same with maternal fish throughout the genome.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 62 (6), 891-896, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282681388654592
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- NII論文ID
- 10004850139
- 10004850772
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- NII書誌ID
- AN00193422
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- COI
- 1:CAS:528:DyaK28Xns1ahurs%3D
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- ISSN
- 1349998X
- 00215392
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- NDL書誌ID
- 4078368
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
