魚類肝臓の可溶性画分におけるフェノール硫酸抱合酵素の活性

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書誌事項

タイトル別名
  • Phenol-sulfate conjugating activity in liver-soluble fraction of fish.
  • 魚類肝臓の可溶性画分におけるフェノール硫酸抱合酵素の活性〔英文〕
  • ギョルイ カンゾウ ノ カヨウセイ カクブン ニ オケル フェノール リュウサ

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説明

The phenol-sulfate conjugatin by the liver subcellular fractions from goldfish Carassius auratus, carp Cyprinus carpio, rainbow trout Salmo gairdneri, and Tilapia nilotica has been studied using [35S] K2SO4. The cell fractination of goldfish liver was examined by the determinatin of respective markers for nuclear, mitochondrial, lysosomal, microsomal, and soluble fractions. Among those subcellular fractions, only the soluble fractin displayed the conjugating activityl The maximum activity of the exzyme in teh soluble fraction was obtained in the presence of 20 μmol of both ATP and Mg2+ in 1.5 ml of medium, although the activity decreased with an excess of ATP and Mg2+. This conjugatin was inhibited by more than 0.1 μmol of Ca2+ in 1.5 ml of medium. The enzyme exhibited the conjugating activity in the pH range 7.5-9.5; the optimum value was at 8. In the temperature range 5-40°C, the maximum conjugation in goldfish, Tilapia, and carp occurred at 35°C, in rainbow trout it occurred at 30°C. The phenol-sulfate conjugating activities in goldfish, carp, rainbow trout, Tilapia and rat were approximately 340, 82, 66, 200 and 720 nmol/g-liver per h, respectively.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 48 (3), 433-437, 1982

    公益社団法人 日本水産学会

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