ＤＮＡフィンガープリントとアイソザイムパターンによるギンブナクローンの識別
書誌事項
- タイトル別名
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- Identification of Clones of Ginbuna Carassius langsdorfii by DNA Fingerprinting and Isozyme Pattern.
- DNA フィンガー プリント ト アイソザイム パターン ニヨル ギンブナクロ
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説明
DNA fingerprinting and isozyme analyses of 32 Ginbuna (all female triploids) collected from the Monobe River, Kochi prefecture were carried out for identification of clones.<br>Genomic DNA extracted from blood were digested with Hae III restriction endonuclease, and were hybridized with 32P-labeled YNZ 22 probe for DNA fingerprinting. Isozymes of muscle and liver frozen at -20°C were assayed by the horizontal starch gel electrophoresis to detect creatine kinase (CK), esterase (EST), glucosephosphate isomerase (GPI), phosphoglucomutase (PGM), general protein (PROT).<br>Specimens were classified into 7 clonal groups by DNA fingerprinting. The mean of band sharing index (BSI) were 0.314±0.330 between individuals, 1.000 between individuals within a clone, and 0.149±0.077 between different clonal lines. The isozyme patterns were also useful for identification of the clones detected by DNA fingerprinting. There were no significant differences in morphological characters between clones of Ginbuna except two morphological traits.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 62 (5), 747-753, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282681389270144
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- NII論文ID
- 10004849802
- 10004850149
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- NII書誌ID
- AN00193422
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- COI
- 1:CAS:528:DyaK28XmtFeltrY%3D
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- ISSN
- 1349998X
- 00215392
- http://id.crossref.org/issn/00215392
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- NDL書誌ID
- 4029272
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- IRDB
- NDLサーチ
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
