Development of 16S rRNA targeted PCR for the identification of Vibrio spp., the causative bacteria of the disease in cultured sea urchin Strongylocentrotus intermedius occurring at low water temperatures.
Bibliographic Information
- Other Title
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- ウニ病害原因菌Vibrio spp.の同定のための16S rRNA遺伝子を標的にした特異的PCRの開発
- ウニ ビョウガイ ゲンインキン Vibrio spp ノ ドウテイ ノ タメ ノ 16S rRNA イデンシ オ ヒョウテキ ニ シタ トクイテキ PCR ノ カイハツ
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Abstract
The causative bacteria, Vibrio spp., have been isolated from the diseased sea urchin Strongylocentrotus intermedius at low water temperatures. To develop a specific identification method for the pathogens, species-specific nucleotide sequences in the 16S rRNA of Vibrio spp., were determined. We designed two PCR primers, DA2F and SKIP, based on the nucleotide sequences : DA2F forward primer (nucleotide numbers 456 to 476 in Fscherichia coli 16S rRNA) for Date isolates and Sk1F forward primer (nucleotide numbers 455 to 478 in E. coli 16S rRNA) for Shiriuchi and Shikabe isolates. The PCR product with about 1kbp was obtained in all causative bacteria from Date, Shiriuchi, Shikabe Breeding Centers and some of Vibrio reference strains by amplification with DA2F-1540R primer set, however, those from three Breeding Centers differed from the reference strains in the case of growth at 30°C. PCR amplification with SK1F-1540R primer set was able to differentiate the causative bacteria from Date and those from Shiriuchi and Shikabe. These results indicate that PCR amplification with two primers is useful for identification of Vibrio spp., which are the pathogens of cultured sea urchin.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 68 (2), 201-206, 2002
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390282681389398912
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- NII Article ID
- 110003145468
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- NDL BIB ID
- 6115157
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed