Different Processes of Pronuclear Events in Pressure-treated and CB-treated Zygotes at the Second Meiosis in Scallop.
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- Komaru Akira
- National Research Institute of Aquaculture
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- Wada Katsuhiko T.
- National Research Institute of Aquaculture
Bibliographic Information
- Other Title
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- ヒオウギガイ受精卵の前核形成過程に及ぼす減数分裂時の水圧処理およびサイトカラシンB処理の影響
- ヒオウギガイ受精卵の前核形成過程に及ぼす減数分裂時の水圧処理およびサイトカラシンB処理の影響〔英文〕
- ヒオウギガイ ジュセイラン ノ ゼンカク ケイセイ カテイ ニ オヨボス ゲン
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Abstract
To examine the effect of cytochalasin B(CB) and hydrostatic pressure treatments on zygotes in Chlamys nobilis for induction of triploidy, we compared the pronuclear events in pressure-treated and CB-treated zygotes at the second meiosis by fluorescence microscopy.<br> The second polar body formation of zygotes was inhibited by treating with CB or hydropressure. In CB-treated zygotes chromosomes were segregated at the second meiosis and developed to two female pronuclei. On the other hand, in pressure-treated zygotes segregation of chromosomes was inhibited and one large diploid female pronucleus was formed. These results suggest CB inhibited only cytokinesis (polar body formation) but pressure inhibited both karyokinesis (segre-gation of chromosomes) and cytokinesis. Although pronuclear events in pressure- and CB-treated zygotes were different, chromatin of pronuclei was condensed normally and became the metaphase chromosome for the first cleavage. These zygotes were thought to deveolp into triploids.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 57 (7), 1219-1223, 1991
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390282681389509760
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- NII Article ID
- 130001545366
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- NDL BIB ID
- 3735373
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed