コイ筋原繊維の熱変性と溶解に及ぼすグルコン酸ナトリウムの影響

書誌事項

タイトル別名
  • Effects of Sodium Gluconate on Thermal Denaturation and Solubilization of Carp Myofibrils.
  • コイ キン ゲン センイ ノ ネツ ヘンセイ ト ヨウカイ ニ オヨボス グルコンサン ナトリウム ノ エイキョウ

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説明

The effects of sodium gluconate (Na-gluconate) on thermal inactivation of Ca-ATPase and solubilization of carp myofibrils were compared with those of sorbitol and sodium chloride (NaCl). Na-gluconate decreased the thermal inactivation rate constant for myofibrillar Ca-ATPase (kD) the same as sorbitol did. On the contrary, NaCl accelerated thermal denaturation of myofibrils. The protective effect (E value) of Na-gluconate on heat-denaturation was considerably larger than that of sorbitol. Although sorbitol had no effect on the solubilization of myofibril, the addition of 0.25-0.30M Na-gluconate induced a decrease in turbidity of myofibril suspension accompanied by the increase in the amount of extracted protein. Therefore, it was clearly demonstrated that Na-gluconate solubilized myofibrils at higher concentration than NaCl. The measurement of sodium ion concentration in the solution with a sodium ion electrode indicated less electrolytic dissociation degree of Nagluconate than NaCl, suggesting that the myofibrils solubilizing effect of both compounds corresponded to their ionization degree in the solution.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 65 (5), 886-891, 1999

    公益社団法人 日本水産学会

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