ウナギ表皮におけるβ-N-アセチルヘキソサミニダーゼの組織化学的検出

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タイトル別名
  • Histochemical localization of .BETA.-N-acetylhexosaminidase in the epidermis of eel.
  • ウナギ表皮におけるB-N-アセチルヘキソサミニダーゼの組織化学的検出〔英文〕
  • ウナギ ヒョウヒ ニ オケル B N アセチルヘキソサミニダーゼ ノ ソシキ

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抄録

To elucidate the origin of β-N-acetylhexosaminidase which had been found in the external mucous materials of varlous fishes, histochemical detection of β-N-acetylhexosaminidase activlty in the epidermis of eel was attempted using naphthol AS-BI N-acetyl, β-D-glucosaminide as sub-strafe and hexazonium pararosanilin as capture reagent. Besides, the structure of eel skin was observed histochemlcally by staining with periodate-Schiff reagent and toluidine blue. β-N-Acetylhexosaminidase activity was demonstrated as red-stained granules around the mature goblet cells in the upper layer of the epidermis. From the fact that the cell membrane in the epidermis was stained with periodate-Schiff reagent and toluidine blue, the membrane was revealed to con-tain carbohydrate components, presumably complex carbohydrates. From the above findings, it was assumed that β-N-acetylhexosaminidase might be involved in catabolic collapse of the super-ficial cells of the epidermis.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 53 (7), 1251-1255, 1987

    公益社団法人 日本水産学会

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