ハナカジカ仔魚の直腸上皮細胞による西洋ワサビペルオキシダーゼの細胞内消化過程の電顕的観察

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  • An ultrastructural study of intracellular digestion of horseradish peroxidase by the rectal epithelium cells in larvae of a freshwater cottid fish Cottus nozawae.
  • ハナカジカ仔魚の直腸上皮細胞による西洋ワサビペルオキシダーゼの細胞内消化過程の電顕的観察〔英文〕
  • ハナカジカ シギョ ノ チョクチョウ ジョウヒ サイボウ ニ ヨル セイヨウ

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The process of ingestion and intracellular digestion of protein molecules by the rectal epithelium cells in larvae of a freshwater cottid fish Cottus nozawae was observed ultrastructurally using horseradish peroxidase (HRP) as a marker protein. Orally administered HRP adhered to the luminal surface of the intermicrovillous plasma membrane of the cell. The membrane loaded with HRP molecules invaginated and was pinched off forming small pinocytotic vesicles. Ingested HRP was transported toward the nucleus by migration of the vesicles. The vesicles grew into large supranuclear vacuoles through repeated fusions thus concertrating the internal HRP. HRP was then accumulated as large homogenous masses in the vacuoles. At this stage, small primary lysosomal bodies came to fuse with the supranuclear vaculoes. and digestion of the HRP masses began form the periphery. With the progress of digestion, a single large mass of HRP in the supranuclear vaculoe was divided into several small bodies, each of which became bounded by a menbrane while its enzymatic activity decreased. Vesicles and vacuoles finally disappeared from the supranuclear area of the cell. No release of HRP into the interellular spaces or basal lamina was obseved. The overall process indicated nutritional signficance of the rectal epithelium cells in protein digestion in the larval stage.

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  • 日本水産学会誌

    日本水産学会誌 50 (3), 409-416, 1984

    公益社団法人 日本水産学会

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