Evaluation of the estrogenic activity in sewage treatment works using a medaka vitellogenin assay

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  • メダカビテロジェニンアッセイによる下水高度処理のエストロゲン様物質低減効果の評価
  • メダカ ビテロジェニン アッセイ ニ ヨル ゲスイ コウド ショリ ノ エストロゲンヨウ ブッシツ テイゲン コウカ ノ ヒョウカ

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Abstract

  The aim of the present study was to determine whether tertiary treatment is effective for decreasing estrogenic activity in a sewage treatment works (STW), using a medaka vitellogenin (VTG) assay. We introduced male Japanese medaka Oryzias latipes into the STW at several points in the treatment process for 2 weeks at a time. Plasma and hepatic VTG levels of the medaka were then determined using an enzyme-linked immunosorbent assay (ELISA) to measure the estrogenic response. Exposure after Activated Sludge Treatment (AS) resulted in elevated plasma and hepatic VTG levels (plasma max., 543 μg/mL; liver max., 26 μg/g wet), close to the levels of females (plasma average, 1028 μg/mL; liver average, 154 μg/g wet). In contrast, VTG was not detected (detection limit: plasma, 0.4 μg/mL; liver, 0.1 μg/g wet) after Stones Contact Oxidization treatment (SC). Furthermore, exposure for 2 weeks activated VTG synthesis more than exposure for 1 week. The plasma and hepatic VTG concentrations correlated well. In addition, we also determined aquatic 17β-estradiol and estrone by ELISA, and found them to be treated more efficiently by SC than AS. Consequently, more effective processing is possible when using both AS and SC.<br>

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