Studies on the metabolism of chlorophenols in fish. XI. Isolation and identification of a conjugated-PCP excreted in the urine of goldfish.

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Other Title
  • ワキンの尿中に排泄されたPCP抱合体の単離と同定
  • ワキンの尿中に排泄されたPCP抱合体の単離と同定〔英文〕
  • ワキン ノ ニョウチュウ ニ ハイセツサレタ PCP ホウゴウタイ ノ タンリ

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Abstract

The renal excretion of PCP absorbed by goldfish, Carassius auratus, exposed to PCP and the isolation and identification of a PCP-conjugate found in the urine have been studied.<br> Approximately 90% of the PCP excreted in the urine was found in a conjugate-form. The conjugate was isolated by treating the urine with an activated charcoal column, followed by elution with an acetone-ammonia mixture, and finally by passing the concentrated eluate through a Sephadex G-10 column.<br> The isolated conjugate was identified as pentachlorophenylsulfate by the elution pattern on the Sephadex column, by thin-layer chromatography, and finally by determination of the molar ratio of PCP to SO4. No other conjugates, such as the glucuronide, were detected in the urine.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 45 (8), 1001-1003, 1979

    The Japanese Society of Fisheries Science

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