海洋性硝酸化成細菌に関する研究-I

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書誌事項

タイトル別名
  • STUDIES ON MARINE NITRIFYING BACTERIA (NITRITE FORMERS AND NITRATE FORMERS) -I
  • カイヨウセイ ショウサン カセイ サイキン ニ カンスル ケンキュウ 1
  • ON THE METHOD OF CULTIVATION AND ON THE DISTRIBUTION (PRELIMINAPY REPORT)
  • 培養法ならびに分布について(予報)

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説明

Preliminary studies were made on the method of cultivation for marine nitrifying bacteria and on the distribution of them.<br> A. Some results on the methods of cultivation were obtained, as follows;<br> 1. Marine nitrifying bacteria don't grow in the ordinary media for soil ones, and grow hardly even in the media prepared by using sea water instead of fresh water.<br> 2. Marine nitrifying bacteria grow well in the medium prepared as follows: (NH4)2SO4; 30mg (for nitrite formers) or KNO2; 30mg (for nitrate formers), KH2PO4; 2mg, and chelated iron (EDTA-Fe); 0.2mg are added to 1 litter of sea water, and this solution is adjusted to pH=8.4 by the addition of 50% K2CO3, and then autoclaved. This medium is very low in the concentration of (NH4)2SO4 or KNO2, comparing with the media for nitrifying bacteria of soil, that is, the concentration of the former is about 1/100 of that of the latter.<br> 3. Further marine nitrifying bacteria grow very well, when small amounts of sand are added into the above mentioned media.<br> B. Researches on the distribution were made by using of the method of cultivation as described above, and some results were obtained, as follows:<br> 1. In the sea water in nature, there are very few nitrite formers and nitrate ones, i. e. both of them are similarly fewer than 0.3cell/ml. In the sea bottom muds, they are the maximum in bacterial counts at the surface layer of the muds, i. e. both of them are similarly 103-104cells/g, and they decrease with the length distant from the surface layer, i. e. are fewer than 102cells/g at the layer bellow 15cm in depth from the surface one.<br> 2. In the breeding sea water in the aquariums of circulating system, nitrite formers and nitrate formers are markedly more than those in the sea water in nature, i. e. both of them are similarly 102-103cells/ml.<br> 3. In the filter-sand of the aquariums of circulating system, both of nitrite formers and nitrate formers are similarly 104-105cells/g and these counts are almost unchanged by the length from the surface layer.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 27 (6), 593-597, 1961

    公益社団法人 日本水産学会

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