Liposome-Assisted Refolding of Microbial Transglutaminase

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  • Umakosh Hiroshi
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Yoshimoto Noriko
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Yoshimoto Makoto
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Shimanouchi Toshinori
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • Kuboi Ryoichi
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

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The liposome-assisted refolding of recombinant microbial transglutaminase (MTG) was achieved by using the dilution method. The analysis of the partly-denatured MTG by using the calcein-trapping immobilized liposome chromatography showed the interaction between the MTG and liposome under the above condition. The effect of the addition of liposome was then investigated, resulting that the refolding yield of MTG was improved from 66% at control to 82% by adding the liposome with suitable membrane fluidity and surface charge density.

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  • 膜

    膜 32 (5), 287-293, 2007

    日本膜学会

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