Preparation of a Poly (L-Lactic Acid) Membrane Scaffold with Open Finger-Like Pores Prepared by a Nonsolvent-Induced Phase Separation Method with the Aid of a Surfactant

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  • 【Original Contribution】 Preparation of a Poly(L–Lactic Acid) Membrane Scaffold with Open Finger–Like Pores Prepared by a Nonsolvent–Induced Phase Separation Method with the Aid of a Surfactant

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Here, we prepared a poly(L–lactic acid) (PLLA) microporous membrane with open finger–like pores as a scaffold for tissue engineering by a nonsolvent–induced phase separation method with the aid of a surfactant. The increase of surfactant content in the polymer solution caused the mold (glass plate) side of the membrane to adopt an indented structure with open finger–like pores to enhance the internal surface area. Osteoblast–like cells inoculated on the indented side grew on and in the PLLA membrane scaffold to reach 2 ～ 3 times higher cell density per unit apparent area compared to that attained in monolayer cultures. The cells on the membrane deposited calcium compounds by osteoinduction with ascorbic acid 2–phosphate, dexamethasone, and β– glycerophosphate. The PLLA membrane with open finger–like pores will likely be useful as scaffolds to support the implantation of osteogenic cells in bone tissue engineering.

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  • 膜

    膜 41 (6), 304-310, 2016

    日本膜学会

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