書誌事項
- タイトル別名
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- Image Analysis of Membrane Receptors in Living Cells by Coiled-coil Labeling Method
- コイルドコイルラベルホウ オ モチイタ マク ジュヨウタイ ノ イメージング カイセキ
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説明
To overcome difficulties of conventional fusion with fluorescent/luminescent proteins, post-translational labeling methods using pairs of small genetically-encodable ‘tags’ and synthetic ‘probes’ targeting the tags have been widely studied in recent years. We have developed a quick tag-probe labeling method using a high-affinity heterodimeric coiled-coil formation between the E3 tag (EIAALEK)3 attached to the target protein and the Kn probe (KIAALKE)n (n = 3 or 4) labeled with a fluorophore. The labeling is cell-surface-specific and completed within 1 min, therefore suitable for monitoring oligomerization/internalization of membrane proteins on living cell surface. Taking advantage of easiness in multicolor labeling, the degree of internalization of the β2 adrenergic receptors following agonist stimulation was evaluated by ratiometric detection of pH decrease in endosomes.
収録刊行物
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- 膜
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膜 38 (2), 82-86, 2013
日本膜学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282681398911872
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- NII論文ID
- 10031165412
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- NII書誌ID
- AN0023215X
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- COI
- 1:CAS:528:DC%2BC3sXhtVaht7fN
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- ISSN
- 18846440
- 03851036
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- NDL書誌ID
- 024608543
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
