Production and characterization of monoclonal antibodies against Actinobacillus (Haemophilus) pleuropneumoniae serotype 2.
Bibliographic Information
- Other Title
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- Actinobacillus (Haemophilus) pleuropneumoniae血清型2・菌株に対するモノクローナル抗体の作製とその性状
- Actinobacillus(Haemophilus)pleuropneumoniae血清型2・菌株に対するモノクローナル抗体の作製とその性状〔英文〕
- Actinobacillus Haemophilus pleuropneumo
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Description
Four hybridoma cell lines producing monoclonal antibodies (MAbs) against Actinobacillus (Haemophilus) pleuropneumoniae were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with a serotype 2, strain SH-15. Enzyme-linked immunosorbent assay-inhibition tests with antigens obtained from 12 serotype strains of A. pleuropneumoniae and 9 other gram-negative bacteria showed that all the MAbs bound to only serotype 2 strains of A. pleuropneumoniae. The epitopes recognized by the MAbs were located on a carbohydrate moiety of lipopolysaccharide (LPS) of the organism, which was sensitive to periodate oxidation. In immunoblotting analyses of LPS obtained from A. pleuropneumoniae serotype 2, all the four MAbs reacted specifically with the characteristic ladder bands of LPS detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results suggest that O-antigen side chains of the LPS are one of the antigenic determinants responsible for the serotype-specificity of A. pleuropneumoniae.
Journal
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- The Japanese Journal of Veterinary Science
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The Japanese Journal of Veterinary Science 52 (3), 533-542, 1990
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Keywords
Details 詳細情報について
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- CRID
- 1390282681399009408
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- NII Article ID
- 110003918227
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- NII Book ID
- AN00191788
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- COI
- 1:STN:280:By%2BA2cfgsFc%3D
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- ISSN
- 18811442
- 00215295
- http://id.crossref.org/issn/00215295
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- NDL BIB ID
- 3670891
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- PubMed
- 2385034
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed